Separation of Polyunsaturated Triglycerides by Column Chromatography on Silicic Acid
نویسندگان
چکیده
منابع مشابه
Separation of tissue cholesterol esters and triglycerides by silicic acid chromatography.
bUnequivocal class separations of tissue lipids are important particularly whcn high temperature gas Chromatography is used at the same time for the determination of the long-chain acid composition of major lipid classes. This problem was studied most recently by Hirsch and Ahrens (1) ; their procedurc involves silicic acid column chromatography with clution by ether-petroleum ether mixtures fo...
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A procedure has been developed for the separation of the glycosyl diglycerides from the phosphatides of Gram-positive bacteria on columns of silicic acid. The method utilizes mixtures of acetone in chloroform for elution of the glycosyl diglycerides, followed by increasing amounts of methanol in chloroform for elution of the phosphatides. The course of the fractionation was followed by means of...
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The parameters affecting the separation and quantification of triglycerides by gas-liquid chromatography have been investigated with the use of QF-1 and SE-30 as stationary phases and a flame ionization detector. The isothermal characteristics of a wide variety of triglycerides (carbon number 6 to 60) on both columns show that long retention volume is directly proportional to carbon number and ...
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The isolation and identification of the phosphonolipids from beef brain--right lobe, left lobe and cerebellum--is herein reported. The phosphonolipids were isolated by preparative thin layer chromatography (TLC) using the solvent system methanol/water (2:1, v/v) and identified preliminarily by TLC. The isolated phosphonolipids were subjected to silicic acid column chromatographic separation and...
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A method is presented for the determination of cholesterol and phospholipid, which requires 5 I . of human serum or 1-2 ml. of cerebrospinal fluids. With this method 5-100 zg. of cholesterol and phospholipid can be separated by a modified silicic acid column after elution of the mixture with 1 ml. of chloroform and 3 ml. of methanol. Recovery for 24.6 sg. of cholesterol and 30.5 g. of phospholi...
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ژورنال
عنوان ژورنال: NIPPON SUISAN GAKKAISHI
سال: 1991
ISSN: 0021-5392,1349-998X
DOI: 10.2331/suisan.57.2159